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Integración cromosomal de HHV6

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El herpesvirus humano 6 (HHV-6) es un virus betaherpes cuyo genoma puede integrarse en los cromosomas humanos. El HHV-6 integrado cromosómicamente (ciHHV-6) puede transmitirse verticalmente de padres a hijos. Se detectó ADN de HHV-6 en el semen, pero su estado integrado o extracromosómico aún no se ha caracterizado. El objetivo de este estudio fue determinar la prevalencia del ADN del HHV-6 y buscar formas de ciHHV-6 en espermatozoides purificados del semen obtenido de sujetos explorados para baja fertilidad. Un total de 184 muestras de esperma se purificaron usando PureSperm®. La carga viral HHV-6 y la identificación de especies se realizaron por reacción en cadena de la polimerasa en tiempo real. De 179 muestras de esperma analizadas, tres fueron positivas para HHV ‐ 6 (1.7%). Dos muestras (1.1%) tenían cargas virales de 680 232 y 2 834 075 copias por millón de espermatozoides, compatibles con las cargas esperadas para un formulario ciHHV-6. La carga viral de la tercera muestra pos…

Estimating the prevalence at death of CTE neuropathology among professional football players

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In July 2017,JAMApublished results from autopsies of the brains of 202 deceased American football players.1Of the 111 participants who had played in the National Football League (NFL), 110 (99%) received a neuropathologic diagnosis of chronic traumatic encephalopathy (CTE), a progressive neurodegenerative disease associated with head trauma. This 99% figure received substantial media coverage when the study was published. REFERENCE; Binney ZO, Bachynski KE. Estimating the prevalence at death of CTE neuropathology among professional football players. Neurology. 2018 Nov 28. pii:  10.1212/WNL.0000000000006699. doi: 10.1212/WNL.0000000000006699. [Epub ahead of print] PubMed PMID: 30487144. Commented on: InsideScience: What's the Risk of Chronic Traumatic Encephalopathy for NFL Players? New study suggests at least one in 10 NFL players could eventually develop the brain disease.

TWiV 453: Neurovirology with Diane Griffin

8º Curso de Animales de Laboratorio #INR 12-14/SEP/2018

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A "Driver Switchover" Mechanism of Influenza Virus Transport from Microfilaments to Microtubules.

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When infecting host cells, influenza virus must move on microfilaments (MFs) at the cell periphery and then move along microtubules (MTs) through the cytosol to reach the perinuclear region for genome release. But how viruses switch from the actin roadway to the microtubule highway remains obscure. To settle this issue, we systematically dissected the role of related motor proteins in the transport of influenza virus between cytoskeletal filaments in situ and in real-time using quantum dot (QD)-based single-virus tracking (SVT) and multicolor imaging. We found that the switch between MF- and MT-based retrograde motor proteins, myosin VI (myoVI) and dynein, was responsible for the seamless transport of viruses from MFs to MTs during their infection. After virus entry by endocytosis, both the two types of motor proteins are attached to virus-carrying vesicles. MyoVI drives the viruses on MFs with dynein on the virus-carrying vesicle hitchhiking. After role exchanges at actin-microtubul…

Inhibition of Retrograde Transport Limits Polyomavirus Infection In Vivo

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Polyomaviruses (PyVs) silently infect most humans, but they can cause life-threatening diseases in immunocompromised individuals. The JC polyomavirus (JCPyV) induces progressive multifocal leukoencephalopathy, a severe demyelinating disease in multiple sclerosis patients receiving immunomodulatory therapy, and BK polyomavirus (BKPyV)-associated nephropathy is a major cause of kidney allograft failure. No effective anti-PyV agents are available. Several compounds have been reported to possess anti-PyV activity in vitro, but none have shown efficacy in clinical trials. Productive PyV infection involves usurping the cellular retrograde vesicular transport pathway to enable endocytosed virions to navigate to the endoplasmic reticulum where virion uncoating begins. Compounds inhibiting this pathway have been shown to reduce infection by simian virus 40 (SV40), JCPyV, and BKPyV in tissue culture. In this study, we investigated the potential of Retro-2.1, a retrograde transport inhibitor, t…

Anterograde transport of rabies virus in DRG neurons.

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Rabies virus (RABV) spread is widely accepted to occur only by retrograde axonal transport. However, examples of anterograde RABV spread in peripheral neurons such as dorsal root ganglion (DRG) neurons indicated a possible bidirectional transport by an uncharacterized mechanism. Here, we analyzed the axonal transport of fluorescence-labeled RABV in DRG neurons by live-cell microscopy. Both entry-related retrogradetransport of RABV after infection at axon endings and postreplicative transport of newly formed virus were visualized in compartmentalized DRG neuron cultures. Whereas entry-related transport at 1.5 μm/s occurred only retrogradely, after 2 days of infection, multiple particles were observed in axons moving in both the anterograde and retrograde directions. The dynamics of postreplicative retrogradetransport (1.6 μm/s) were similar to those of entry-related retrogradetransport. In contrast, anterograde particle transport at 3.4 μm/s was faster, indicating active particle tran…